Organic Residue Analysis
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Recent papers in Organic Residue Analysis
The application of biomolecular techniques to archaeological materials from the Balkans is providing valuable new information on the prehistory of the region. This is especially relevant for the study of the neolithisation process in SE... more
The application of biomolecular techniques to archaeological materials from the Balkans is providing valuable new information on the prehistory of the region. This is especially relevant for the study of the neolithisation process in SE Europe, which gradually affected the rest of the continent. Here, to answer questions regarding diet and subsistence practices in early farming societies in the central Balkans, we combine organic residue analyses of archaeological pottery, taxonomic and isotopic study of domestic animal remains and biomolecular analyses of human dental calculus. The results from the analyses of the lipid residues from pottery suggest that milk was processed in ceramic vessels. Dairy products were shown to be part of the subsistence strategies of the earliest Neolithic communities in the region but were of varying importance in different areas of the Balkan. Conversely, milk proteins were not detected within the dental calculus. The molecular and isotopic identification of meat, dairy, plants and beeswax in the pottery lipids also provided insights into the diversity of diet in these early Neolithic communities, mainly based on terrestrial resources. We also present the first compound-specific radiocarbon dates for the region, obtained directly from absorbed organic residues extracted from pottery, identified as dairy lipids.
The application of biomolecular techniques to archaeological materials from the Balkans is providing valuable new information on the prehistory of the region. This is especially relevant for the study of the neolithisation process in SE... more
The application of biomolecular techniques to archaeological materials from the Balkans is providing valuable new information on the prehistory of the region. This is especially relevant for the study of the neolithisation process in SE Europe, which gradually affected the rest of the continent. Here, to answer questions regarding diet and subsistence practices in early farming societies in the central Balkans, we combine organic residue analyses of archaeological pottery, taxonomic and isotopic study of domestic animal remains and biomolecular analyses of human dental calculus. The results from the analyses of the lipid residues from pottery suggest that milk was processed in ceramic vessels. Dairy products were shown to be part of the subsistence strategies of the earliest Neolithic communities in the region but were of varying importance in different areas of the Balkan. Conversely, milk proteins were not detected within the dental calculus. The molecular and isotopic identification of meat, dairy, plants and beeswax in the pottery lipids also provided insights into the diversity of diet in these early Neolithic communities, mainly based on terrestrial resources. We also present the first compound-specific radiocarbon dates for the region, obtained directly from absorbed organic residues extracted from pottery, identified as dairy lipids.
In this chapter, we present comparative analyses of artifacts and ecofacts from most of the Project excavations at Nebelivka (House A9, the Mega-structure, the Pit in Sondazh 1 and the test pits). The finds from the Ukrainian excavations... more
In this chapter, we present comparative analyses of artifacts and ecofacts from most of the Project excavations at Nebelivka (House A9, the Mega-structure, the Pit in Sondazh 1 and the test pits). The finds from the Ukrainian excavations of Houses B17 and B18, the 'industrial feature' and their respective pits are published elsewhere. All classes of finds were subject to the same taphonomic protocols before comparative analysis between the excavation units at Nebelivka and comparisons with other Trypillia sites and megasites. A team of pottery specialists considered alternatives to the Ryzhov pottery system, using the sherd rather than the whole vessel as the unit for 14 different comparative analyses. Dmytro Kiosak examined the small lithic assemblage, identifying a major decrease in lithic deposition after the large Early Trypillia samples. The special finds analysis considered the sample of almost 100 figurines, fired clay tokens and the only gold ornament known so far from the Trypillia group. David Orton and colleagues have written the first modern faunal report of a Trypillia assemblage, paying attention to inter-analyst variability and contextual variability. The small botanical assemblage, discussed by Galyna Pashkevych, was the result of the first water-sieving operation conducted on a Trypillia excavation and confirmed her views, counterfactual for megasites, on Trypillia arable farming as low in production and efficiency.
The earliest evidence for cheese production in the Mediterranean is revealed by stable carbon isotope analyses of individual fatty acids in pottery residues from the Dalmatian coast of Croatia. Lipid residue data indicate the presence of... more
The earliest evidence for cheese production in the Mediterranean is revealed by stable carbon isotope analyses of individual fatty acids in pottery residues from the Dalmatian coast of Croatia. Lipid residue data indicate the presence of milk in the earliest pottery, Impressed Ware, by 5700 cal. BCE (7700 BP). In contrast, by 5200 cal BCE (7200 BP), milk was common in refined Figulina pottery, meat was mostly associated with Danilo ware, cheese occurred in Rhyta, and sieves contained fermented dairy, representing strong links between specific function and stylistically distinctive pottery vessels. Genetic data indicate the prevalence of lactose intolerance among early farming populations. However, young children are lactase persistent until after weaning and could consume milk as a relatively pathogen-free and nutrient rich food source, enhancing their chances of survival into adulthood. Fermentation of milk into yogurt and cheese decreases lactose content. The evidence for fermented dairy products by 5200 cal BCE indicates a larger proportion of the population was able to consume dairy products and benefit from their significant nutritional advantages. We suggest that milk and cheese production among Europe's early farmers reduced infant mortality and helped stimulate demographic shifts that propelled farming communities to expand to northern latitudes.
- by Emil Podrug and +1
- •
- Neolithic, Organic Residue Analysis
Between 2800 and 2400 cal BC pastoralists from Central Europe migrated into the eastern Baltic paving the way for the Corded Ware Culture (CWC), and a new type of economy, animal husbandry. Traditionally the CWC people were viewed as... more
Between 2800 and 2400 cal BC pastoralists from Central Europe migrated into the eastern Baltic paving the way for the Corded Ware Culture (CWC), and a new type of economy, animal husbandry. Traditionally the CWC people were viewed as highly mobile due to the lack of substantial traces of dwellings and material culture at settlement sites; they were reliant on an economy based on animal husbandry as demonstrated by zooarchaeological and stable isotopic evidence. However, this paradigm is beginning to shift. Here, we present new AMS radiocarbon (14C) measurements, pollen and macrobotanical data from sediment samples and a portable fi sh screen, as well as technological, molecular and isotopic data obtained from ceramic vessels from three CWC sites in the eastern Baltic. Overall, our results indicate a de-Neolithisation process undergone by some CWC groups, particularly in lacustrine and coastal ecotones, and a shift to hunting, gathering and fishing.
The first five excavation seasons of the Lautenschläger Azekah Expedition have revealed the long occupational history of the site. A destruction layer dating to the Late Bronze Age was exposed in almost every excavation area of the site,... more
The first five excavation seasons of the Lautenschläger Azekah Expedition have revealed the long occupational history of the site. A destruction layer dating to the Late Bronze Age was exposed in almost every excavation area of the site, enabling various multi-disciplinary studies of a wide range of material remains. This paper focuses on stratigraphic investigations, ceramic analyses, results from a radiocarbon dating project, residue analysis of pottery containers, physical anthropological studies and glyptic and figurative examinations. The results provide testimony to the character of daily life, aspects of interaction with Egyptian overlords, and observable transformations in concepts and consumption practices at Tel Azekah in the Late Bronze Age.
For a long time, the nutritional practices of Ancient Egypt were discussed on the basis of written, pictorial and archaeobotanical sources. Especially the hieratically inscribed vessels of the New Kingdom seemed to be a reliable source... more
For a long time, the nutritional practices of Ancient Egypt were discussed on the basis of written, pictorial and archaeobotanical sources. Especially the hieratically inscribed vessels of the New Kingdom seemed to be a reliable source for the stored and consumed food. At the same time, it has not yet been possible to understand all the terms for foodstuffs. The poster presents the results of our first series of organic residue analyses (ORA) from labelled vessels from Amarna, today in the British Museum. Correlations between inscription and content, but also complex usage biographies and information on trading mechanisms are discussed.
This paper focuses on the functional analysis of a specific oval shaped basin diffused in Lower Egyptian Predynastic sites during the first half of the 4th millennium BC. These oval shallow ceramic basins are characterised by a flat and... more
This paper focuses on the functional analysis of a specific oval shaped basin diffused in Lower Egyptian Predynastic sites during the first half of the 4th millennium BC. These oval shallow ceramic basins are characterised by a flat and wide inner surface that is covered by a layer of small rock fragments pressed into the clay matrix. Several archeologists have interpreted them as grinding tools, but the limited number of samples unearthed thus far and their poor state of preservation made this interpretation uncertain. Here we present the results of a preliminary integrated study based on the combination of experimental archaeology and petrographic, use-wear and residue analyses carried out on two samples from the Predynastic site of Maadi. The use-wear analysis of the archaeological fragments highlighted traces of an intentional grinding and light pounding of oily substances, which is also partially supported by the organic residue investigation. These results were tested through experimental tests that confirmed these basins are most likely mortars.
Establishing the actual functional activities performed in such a unique type of vessel is important not only for understanding the social behaviors of the Lower Egyptian Predynastic communities and the dynamics related to the diffusion of such basins, but it also provides a new methodological framework for Egyptian Predynastic studies.
Establishing the actual functional activities performed in such a unique type of vessel is important not only for understanding the social behaviors of the Lower Egyptian Predynastic communities and the dynamics related to the diffusion of such basins, but it also provides a new methodological framework for Egyptian Predynastic studies.
The paper aims at investigating an unusual pottery category which occurs in the Late Chalcolithic of Southern Levant, as well as in Western Anatolia, Northern Aegean and Sicily between the early Fourth and middle Third Millennium BC. In... more
The paper aims at investigating an unusual pottery category which occurs in the Late Chalcolithic of Southern Levant, as well as in Western Anatolia, Northern Aegean and Sicily between the early Fourth and middle Third Millennium BC. In the archaeological literature of eastern
Mediterranean prehistory cylindrical barrel-shaped vessels are interpreted as “churns”, because they closely resemble skin-bottles used by modern pastoral nomads in rural Anatolia for converting milk into products such as butter or light cheese.
The archaeological investigations and the results of complementary chemical analyses to detect organic residues, involve some ethnographic evidence, in order to define use and function of such uncommon pottery assemblage, which represents one of the most important element of dairy production.
Mediterranean prehistory cylindrical barrel-shaped vessels are interpreted as “churns”, because they closely resemble skin-bottles used by modern pastoral nomads in rural Anatolia for converting milk into products such as butter or light cheese.
The archaeological investigations and the results of complementary chemical analyses to detect organic residues, involve some ethnographic evidence, in order to define use and function of such uncommon pottery assemblage, which represents one of the most important element of dairy production.
The introduction of farming had far-reaching impacts on health, social structure and demography. Although the spread of domesticated plants and animals has been extensively tracked, it is unclear how these nascent economies developed... more
The introduction of farming had far-reaching impacts on health, social structure and demography. Although the spread of domesticated plants and animals has been extensively tracked, it is unclear how these nascent economies developed within different environmental
and cultural settings. Using molecular and isotopic analysis of lipids from pottery, here we investigate the foods prepared by the earliest farming communities of the European Atlantic seaboard. Surprisingly, we find an absence of aquatic foods, including in ceramics from
coastal sites, except in the Western Baltic where this tradition continued from indigenous ceramic using hunter-gatherer-fishers. The frequency of dairy products in pottery increased as farming was progressively introduced along a northerly latitudinal gradient. This finding
implies that early farming communities needed time to adapt their economic practices before expanding into more northerly areas. Latitudinal differences in the scale of dairy production might also have influenced the evolution of adult lactase persistence across Europe.
and cultural settings. Using molecular and isotopic analysis of lipids from pottery, here we investigate the foods prepared by the earliest farming communities of the European Atlantic seaboard. Surprisingly, we find an absence of aquatic foods, including in ceramics from
coastal sites, except in the Western Baltic where this tradition continued from indigenous ceramic using hunter-gatherer-fishers. The frequency of dairy products in pottery increased as farming was progressively introduced along a northerly latitudinal gradient. This finding
implies that early farming communities needed time to adapt their economic practices before expanding into more northerly areas. Latitudinal differences in the scale of dairy production might also have influenced the evolution of adult lactase persistence across Europe.
The introduction of pottery vessels to Europe has long been seen as closely linked with the spread of agriculture and pastoralism from the Near East. The adoption of pottery technology by hunter–gatherers in Northern and Eastern Europe... more
The introduction of pottery vessels to Europe has long been seen as closely linked with the spread of agriculture and pastoralism from the Near East. The adoption of pottery technology by hunter–gatherers in Northern and Eastern Europe does not fit this paradigm, and its role within these communities is so far unresolved. To investigate the motivations for hunter–gatherer pottery use, here, we present the systematic analysis of the contents of 528 early vessels from the Baltic Sea region, mostly dating to the late 6th–5th millennium cal BC, using molecular and isotopic characterization techniques. The results demonstrate clear sub-regional trends in the use of ceramics by hunter–gatherers; aquatic resources in the Eastern Baltic, non-ruminant animal fats in the Southeastern Baltic, and a more variable use, including ruminant animal products, in the Western Baltic, potentially including dairy. We found surprisingly little evidence for the use of ceramics for non-culinary activities, such as the production of resins. We attribute the emergence of these subregional cuisines to the diffusion of new culinary ideas afforded by the adoption of pottery, e.g. cooking and combining foods, but culturally contextualized and influenced by traditional practices.
The introduction of pottery vessels to Europe has long been seen as closely linked with the spread of agriculture and pastoralism from the Near East. The adoption of pottery technology by hunter-gatherers in Northern and Eastern Europe... more
The introduction of pottery vessels to Europe has long been seen as closely linked with the spread of agriculture and pastoralism from the Near East. The adoption of pottery technology by hunter-gatherers in Northern and Eastern Europe does not fit this paradigm, and its role within these communities is so far unresolved. To investigate the motivations for hunter-gatherer pottery use, here, we present the systematic analysis of the contents of 528 early vessels from the Baltic Sea region, mostly dating to the late 6th-5th millennium cal BC, using molecular and isotopic characterization techniques. The results demonstrate clear sub-regional trends in the use of ceramics by hunter-gatherers; aquatic resources in the Eastern Baltic, non-ruminant animal fats in the Southeastern Baltic, and a more variable use, including ruminant animal products, in the Western Baltic, potentially including dairy. We found surprisingly little evidence for the use of ceramics for non-culinary activities, such as the production of resins. We attribute the emergence of these sub-regional cuisines to the diffusion of new culinary ideas afforded by the adoption of pottery, e.g. cooking and combining foods, but culturally contextualized and influenced by traditional practices.
The study of childhood diet, including breastfeeding and weaning, has important implications for our understanding of infant mortality and fertility in past societies1. Stable isotope analyses of nitrogen from bone collagen and dentine... more
The study of childhood diet, including breastfeeding and weaning,
has important implications for our understanding of infant
mortality and fertility in past societies1. Stable isotope analyses of
nitrogen from bone collagen and dentine samples of infants have
provided information on the timing of weaning2; however, little is
known about which foods were consumed by infants in prehistory.
The earliest known clay vessels that were possibly used for feeding
infants appear in Neolithic Europe, and become more common
throughout the Bronze and Iron Ages. However, these vessels—
which include a spout through which liquid could be poured—have
also been suggested to be feeding vessels for the sick or infirm3,4.
Here we report evidence for the foods that were contained in
such vessels, based on analyses of the lipid ‘fingerprints’ and the
compound-specific δ13C and Δ13C values of the major fatty acids of
residues from three small, spouted vessels that were found in Bronze
and Iron Age graves of infants in Bavaria. The results suggest that
the vessels were used to feed infants with milk products derived
from ruminants. This evidence of the foodstuffs that were used to
either feed or wean prehistoric infants confirms the importance of
milk from domesticated animals for these early communities, and
provides information on the infant-feeding behaviours that were
practised by prehistoric human groups.
has important implications for our understanding of infant
mortality and fertility in past societies1. Stable isotope analyses of
nitrogen from bone collagen and dentine samples of infants have
provided information on the timing of weaning2; however, little is
known about which foods were consumed by infants in prehistory.
The earliest known clay vessels that were possibly used for feeding
infants appear in Neolithic Europe, and become more common
throughout the Bronze and Iron Ages. However, these vessels—
which include a spout through which liquid could be poured—have
also been suggested to be feeding vessels for the sick or infirm3,4.
Here we report evidence for the foods that were contained in
such vessels, based on analyses of the lipid ‘fingerprints’ and the
compound-specific δ13C and Δ13C values of the major fatty acids of
residues from three small, spouted vessels that were found in Bronze
and Iron Age graves of infants in Bavaria. The results suggest that
the vessels were used to feed infants with milk products derived
from ruminants. This evidence of the foodstuffs that were used to
either feed or wean prehistoric infants confirms the importance of
milk from domesticated animals for these early communities, and
provides information on the infant-feeding behaviours that were
practised by prehistoric human groups.
The site of Sphinx (SBK.W-60) is located about 3.5 km from the present Nile in the western part of Jebel Sabaloka, upstream of the Sixth Nile Cataract, in Sudan. This site uniquely includes Early Khartoum (Mesolithic) artifacts with no... more
The site of Sphinx (SBK.W-60) is located about 3.5 km from the present Nile in the western part of Jebel Sabaloka, upstream of the Sixth Nile Cataract, in Sudan. This site uniquely includes Early Khartoum (Mesolithic) artifacts with no intrusive elements and has been dated from the ninth to the end of the sixth millennium cal BC. Excavations at Trench 7, in particular, brought to light a 1.2-m thick deposit with the quantitatively and qualitatively richest artifactual materials. Analysis and classification of the pottery assemblage from this site have been conducted with the aim of observing manufacturing techniques and examining the correlation between pottery production, cultural change and chronological variability. We undertook visual examinations of the manufacturing techniques combined with petrographic (optical microscopy, OM) and chemical analyses (instrumental Neutron Activation Analysis, iNAA), observations of manufacturing and decorative techniques, gas chromatography-mass spectrometry (GC-MS) and gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) on absorbed organic residues. The vertical distribution of the ceramic assemblage in Trench 7 reveals the existence of a relative sequence suggesting consistent technological variability throughout the site's occupation.
Volume 2 of the BEFIM series contains six papers by the BEFIM team and close co-operation partners. The interim reports of BEFIM 1 are enhanced, finalised, and integrated into overall interpretations. An introductory paper provides... more
Volume 2 of the BEFIM series contains six papers by the BEFIM team and close co-operation partners. The interim reports of BEFIM 1 are enhanced, finalised, and integrated into overall interpretations. An introductory paper provides insights into and an overview of the potential of biomarker and isotopic analysis in studying ancient organic residues.
The core of the book consists of three articles on the final archaeo-scientific results of BEFIM. The first paper combines the ORA results of local and imported pottery from early, younger, and current excavations on and around the Mont Lassios with excavation contexts and aspects of vessel shapes. By doing so, it becomes possible to compare the use of different vessel shapes and wares in each of the settlement zones. The second contribution integrates the organic residue analyses (ORA) of pottery from the old excavations (1950-85) and recent excavations at both the Vorburg settlement (2004-08) and on the Plateau (2015) of the Heuneburg into their archaeological contexts. The pottery from relevant find contexts allows for a spatially and temporally differentiated view of eating and drinking habits and vessel usage, followed by comparisons with the evidence from the Mont Lassois. The third text presents the results of food residue analyses from Breisach-Münsterberg in their archaeological context.
The volume ends with two gender-historical contributions, one on the question of alcohol in the female sphere of life in ancient cultures of Europe and the Near East with regard to production, distribution, trade, ritual, and consumption, the other one on gender stereotypes in the interpretation of ostentatious Iron Age graves.
The core of the book consists of three articles on the final archaeo-scientific results of BEFIM. The first paper combines the ORA results of local and imported pottery from early, younger, and current excavations on and around the Mont Lassios with excavation contexts and aspects of vessel shapes. By doing so, it becomes possible to compare the use of different vessel shapes and wares in each of the settlement zones. The second contribution integrates the organic residue analyses (ORA) of pottery from the old excavations (1950-85) and recent excavations at both the Vorburg settlement (2004-08) and on the Plateau (2015) of the Heuneburg into their archaeological contexts. The pottery from relevant find contexts allows for a spatially and temporally differentiated view of eating and drinking habits and vessel usage, followed by comparisons with the evidence from the Mont Lassois. The third text presents the results of food residue analyses from Breisach-Münsterberg in their archaeological context.
The volume ends with two gender-historical contributions, one on the question of alcohol in the female sphere of life in ancient cultures of Europe and the Near East with regard to production, distribution, trade, ritual, and consumption, the other one on gender stereotypes in the interpretation of ostentatious Iron Age graves.
What the so-called “early Celts” used to drink has been a matter of debate amongst scientists and in the wider public for more than a century. Finds of Mediterranean imported pottery were the reason for scholars to suspect already at an... more
What the so-called “early Celts” used to drink has been a matter of debate amongst scientists and in the wider public for more than a century. Finds of Mediterranean imported pottery were the reason for scholars to suspect already at an early date that the “Celts” had aimed at imitating Mediterranean drinking habits. Until recently, these drinking vessels of Greek origin and Mediterranean transport amphorae were considered proof of exactly this adoption of Greek lifestyle even north of the Alps. This is the point, where our conference came into play, the basis of which is research conducted in the context of the joint research project BEFIM (www.befim.de).
We aim at connecting innovative approaches from both arts and sciences and at profitably transferring them to the archaeological evidence. To this purpose, academics from entirely different disciplines presented their research results at the conference – whether it be on interculturality, pottery of the early Celts, scientific analyses of food residues or wine consumption also beyond the Keltiké.
In the centre of interest there were questions such as these: Did the contact with foreign drinking customs and the associated vessels unfold transformative potential even beyond the group of persons we simplistically call the “elite”? How quickly and in which contexts were exotic drinks (such as wine in particular) appropriated? Was this accompanied by the use of new vessel types and thus perhaps also by different drinking habits? Did the acceptance of unusual banqueting customs result in cultural change beyond the early Celts? How can we prove the presence of these Mediterranean drinks? What used to be consumed in which way before their arrival? What types of food lent themselves to being transformed into (alcoholic) beverages at all and which techniques for producing drinks are reflected in the archaeological evidence? Is the exchange of knowledge on the production and seasoning of beverages recognisable in the intercultural dialogue?
This volume with its general introductions, overview articles, and specialised papers is directed at a broader academic public from universities, museums, and heritage preservation authorities on the one hand as well as students and interested non-specialists on the other hand.
We aim at connecting innovative approaches from both arts and sciences and at profitably transferring them to the archaeological evidence. To this purpose, academics from entirely different disciplines presented their research results at the conference – whether it be on interculturality, pottery of the early Celts, scientific analyses of food residues or wine consumption also beyond the Keltiké.
In the centre of interest there were questions such as these: Did the contact with foreign drinking customs and the associated vessels unfold transformative potential even beyond the group of persons we simplistically call the “elite”? How quickly and in which contexts were exotic drinks (such as wine in particular) appropriated? Was this accompanied by the use of new vessel types and thus perhaps also by different drinking habits? Did the acceptance of unusual banqueting customs result in cultural change beyond the early Celts? How can we prove the presence of these Mediterranean drinks? What used to be consumed in which way before their arrival? What types of food lent themselves to being transformed into (alcoholic) beverages at all and which techniques for producing drinks are reflected in the archaeological evidence? Is the exchange of knowledge on the production and seasoning of beverages recognisable in the intercultural dialogue?
This volume with its general introductions, overview articles, and specialised papers is directed at a broader academic public from universities, museums, and heritage preservation authorities on the one hand as well as students and interested non-specialists on the other hand.
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