While the solar water disinfection (SODIS) method of treating microbiologically contaminated water at the household level has proven to be effective at reducing incidence of diarrhea, its effectiveness is limited to waters of low turbidity. This study investigates the use of table salt (NaCl) to reduce turbidity in water containing dispersed colloidal clay particles as a means of expanding the user base of SODIS. Jar tests were performed on solutions of a low-activity clay, simulating the general composition of soils of the Vertisol type, which are found in key developing regions. Results show that dispersions exhibited as high as 92% particle removal efficiency. The results of this study suggest that NaCl in combination with as little as 30% bentonite by mass may be used to produce a small-scale jumpstart effect by reducing turbidity to a level suitable for SODIS treatment. Soil type was mapped and overlaid with population estimates in a GIS environment to highlight geographic areas where salt+SODIS may be most viable in the case study of South Sudan. Findings suggest that the NaCl method could expand access to SODIS technology by about 1.56 million people who currently lack access to an improved water source in the case study.

Solar water disinfection (SODIS) has proven to be effective at reducing diarrheal incidence in epidemiological intervention studies. However, the SODIS method is limited to waters of low turbidity (<30 NTU). This study investigates the use of common table salt (NaCl) to reduce the turbidity of water containing suspended colloidal clay particles for use in the SODIS method. Three representative clays found in tropical soils (kaolinite, illite and bentonite) were tested at three levels of turbidity (50, 100 and 200 NTU) for their flocculating behavior with multiple NaCl concentrations to find the optimum. Supernatants were tested for sodium concentration for comparison against health and taste thresholds. Results show that unlike kaolinite and illite, pure bentonite solutions were shown to be very responsive to NaCl and produced supernatants with as low as 4 NTU (98% particle removal efficiency). This study has shown that NaCl, in combination with high-activity clay particles in solution, may effectively reduce turbidity to levels suitable for SODIS treatment, thereby expanding the number of people who can utilize the technology effectively.

A promising approach to reducing the >870,000 deaths/year globally from unsafe water is flow-through solar water pasteurization systems (SWPs). Unfortunately, demonstrated systems have high capital costs, which limits access for the poor. The most expensive component of such systems is the heat exchanger (HX). Thus, this study focuses on cost optimization of HX designs for flow-through SWPs using high-effectiveness polymer microchannel HXs. The theoretical foundation for the cost optimization of a polymer microchannel HX is provided, and outputs are plotted in order to provide guidelines for designers to perform HX optimizations. These plots are used in two case studies: (1) substitution of a coiled copper HX with polymer microchannel HX, and (2) design of a polymer microchannel HX for a 3-D printed collector that can fit in an arbitrary build volume. The results show that substitution of the polymer expanded HX reduced the overall expenditure for the system by a factor 50, which aids in making the system more economical. For the second case study, the results show how future system designers can optimize an HX for an arbitrary SWP geometry. The approach of distributed manufacturing using laser welding appears promising for HX for SWP.

Nitrate, the most oxidized form of nitrogen, is regulated to protect people and animals from harmful levels as there is a large over abundance due to anthropogenic factors. Widespread field testing for nitrate could begin to address the nitrate pollution problem, however, the Cadmium Reduction Method, the leading certified method to detect and quantify nitrate, demands the use of a toxic heavy metal. An alternative, the recently proposed Environmental Protection Agency Nitrate Reductase Nitrate-Nitrogen Analysis Method, eliminates this problem but requires an expensive proprietary spectrophotometer. The development of an inexpensive portable, handheld photometer will greatly expedite field nitrate analysis to combat pollution. To accomplish this goal, a methodology for the design, development, and technical validation of an improved open-source water testing platform capable of performing Nitrate Reductase Nitrate-Nitrogen Analysis Method. This approach is evaluated for its potential to i) eliminate the need for toxic chemicals in water testing for nitrate and nitrite, ii) reduce the cost of equipment to perform this method for measurement for water quality, and iii) make the method easier to carryout in the field. The device is able to perform as well as commercial proprietary systems for less than 15% of the cost for materials. This allows for greater access to the technology and the new, safer nitrate testing technique.

RepRaps (self Replicating Rapid prototypers), which 3-D print objects using fused filament fabrication (FFF), have evolved rapidly since their open source introduction. These 3-D printers have primarily been limited to desktop sizes of volumes of ~8000 cm 3 , which has limited the attention of the scientific community to investigating deformation of common thermoplastics such as polylactic acid (PLA) used in FFF printing. The only existing physically relevant deformation model was expanded here to use a physics-based temperature gradient instead of a step function. This was necessary to generalize the model to 3-D printing in a room temperature environment without a heated chamber. The thermal equation was calibrated using thermal measurements and validated by measuring curvatures in printed objects. The results confirm that this is a valid model for predicting warpage of thin vertical walls of PLA. Additionally, the effect of annealing was examined. It was found that at a temperature of 50°C, no shrinkage or crystallization takes place, but at 90°C the PLA rapidly crystallizes to around 20% crystallinity. This indicates that heated bed temperatures should be maintained at 50 o C or lower to avoid print failure (premature substrate release) with PLA. At 90°C, the annealing is accompanied by a 5% size decrease in both horizontal dimensions, but an 8% increase in the vertical dimension. Thus, the volume decreased by only 3%. This observation may lead to potential methods of improving slicing of printing large PLA objects with FFF.

The rise of Free and Open Source models for software development has catalyzed the growth of Free and Open Source Hardware (also known as " Libre Hardware "). Libre hardware is gaining significant traction in the scientific hardware community, where there is evidence that open development creates both technically superior and far less expensive scientific equipment than proprietary models. In this article, the evidence is reviewed and a collection of examples of business models is developed to service scientists who have the option to manufacture their own equipment using Open Source designs. Profitable Libre Hardware business models are reviewed, which includes kit, specialty component, and calibration suppliers for makers. The results indicate that Libre Hardware businesses should target technically sophisticated customers first and, as usability matures, target expanded markets of conventional consumers.

There is growing evidence that low-cost open-source 3-D printers can reduce costs by enabling distributed manufacturing of substitutes for both specialty equipment and conventional mass-manufactured products. The rate of 3-D printable designs under open licenses is growing exponentially and there are already hundreds of designs applicable to small-scale organic farming. It has also been hypothesized that this technology could assist sustainable development in rural communities that rely on small-scale organic agriculture. To gauge the present utility of open-source 3-D printers in this organic farm context both in the developed and developing world, this paper reviews the current open-source designs available and evaluates the ability of low-cost 3-D printers to be effective at reducing the economic costs of farming.This study limits the evaluation of open-source 3-D printers to only the most-developed fused filament fabrication of the bioplastic polylactic acid (PLA). PLA is a strong biodegradable and recyclable thermoplastic appropriate for a range of representative products, which are grouped into five categories of prints: handtools, food processing, animal management, water management and hydroponics. The advantages and shortcomings of applying 3-D printing to each technology are evaluated. The results show a general technical viability and economic benefit to adopting open-source 3-D printing for any of the technologies, although the individual economic impact is highly dependent on needs and frequency of use on a specific farm. Capital costs of a 3-D printer may be saved from on-farm printing of a single advanced analytical instrument in a day or replacing hundreds of inexpensive products over a year. In order for the full potential of open-source 3-D printing to be realized to assist organic farm economic resiliency and self-sufficiency, future work is outlined in five core areas: designs of 3-D printable objects, 3-D printing materials, 3-Dprinters, software and 3-D printable repositories.

Centrifuges are commonly required devices in medical diagnostics facilities as well as scientific laboratories. Although there are commercial and open source centrifuges, the costs of the former and the required electricity to operate the latter limit accessibility in resource-constrained settings. There is a need for low-cost, human-powered, verified, and reliable lab-scale centrifuges. This study provides the designs for a low-cost 100% 3-D printed centrifuge, which can be fabricated on any low-cost RepRap-class (self-replicating rapid prototyper) fused filament fabrication (FFF)-or fused particle fabrication (FPF)-based 3-D printer. In addition, validation procedures are provided using a web camera and free and open source software. This paper provides the complete open source plans, including instructions for the fabrication and operation of a hand-powered centrifuge. This study successfully tested and validated the instrument, which can be operated anywhere in the world with no electricity inputs, obtaining a radial velocity of over 1750 rpm and over 50 N of relative centrifugal force. Using commercial filament, the instrument costs about U.S. $25, which is less than half of all commercially available systems. However, the costs can be dropped further using recycled plastics on open source systems for over 99% savings. The results are discussed in the context of resource-constrained medical and scientific facilities.

The open-source release of self-replicating rapid prototypers (RepRaps) has created a rich opportunity for low-cost distributed digital fabrication of complex 3-D objects such as scientific equipment. For example, 3-D printable reactionware devices offer the opportunity to combine open hardware microfluidic handling with lab-on-a-chip reactionware to radically reduce costs and increase the number and complexity of microfluidic applications. To further drive down the cost while improving the performance of lab-on-a-chip paper-based microfluidic prototyping, this study reports on the development of a RepRap upgrade capable of converting a Prusa Mendel RepRap into a wax 3-D printer for paper-based microfluidic applications. An open-source hardware approach is used to demonstrate a 3-D printable upgrade for the 3-D printer, which combines a heated syringe pump with the RepRap/Arduino 3-D control. The bill of materials, designs, basic assembly, and use instructions are provided, along with a completely free and open-source software tool chain. The open-source hardware device described here accelerates the potential of the nascent field of electrochemical detection combined with paper-based microfluidics by dropping the marginal cost of prototyping to nearly zero while accelerating the turnover between paper-based microfluidic designs.