Antimicrobial activity of Black Cumin seeds (Nigella sativa) against multidrug resistant strains of Coagulase negative Staphylococci.

Objective: Staphylococcus aureus is one of the major resistant pathogens extremely adaptable to antibiotic pressure. Nigella sativa (black cumin) seed extracts and essential oil have been shown to possess antimicrobial activity against several bacteria but little work has been done on their effect against multidrug resistant S. aureus strains isolated from patients. So, we studied antibacterial activity of Nigella sativa against multidrug resistant clinical strains of Staphylococcus aureus. It was an experimental, in vitro study. Materials and methods: Nigella sativa (black cumin) seed essential oil and extracts were tested in varying dilutions against 40 clinical strains of Staphylococcus aureus which were isolated from patients attending a tertiary care teaching hospital in North India using disc agar diffusion technique on inoculated Muellar Hinton agar plates under standard laboratory conditions. The tested strains were resistant to 4 or more clinically used antibiotics belonging to at least 3 different classes. Results: The Methanolic extract and oil of Nigella sativa were found active against 38 and 35 multi-drug resistant strains respectively. Both the oil and Methanolic extract showed remarkable dose dependant antibacterial activity against the tested strains up to a dilution of 1:50 as evident from the zones of inhibition. Conclusion: Nigella sativa possesses antibacterial activity against multidrug resistant clinical strains of Staphylococcus aureus

Journal of Ayub Medical College, Abbottabad: JAMC

J Ayub Med Coll …, 2008

An alarming increase in bacterial strains resistant to existing antimicrobial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to current antimicrobials. Nigella sativa Linn. (Black cumin) essential oil was studied for antibacterial activity against various clinical isolates of bacteria resistant to a number of antibiotics, in varying concentrations by Disc Agar diffusion technique using impregnated filter paper discs on inoculated Muellar Hinton agar plates. The oil showed pronounced dose dependant antibacterial activity which was more against Gram positive than Gram negative bacteria. Among Gram positive bacteria tested, Staphylococcus aureus, S. epidermidis, other coagulase –ve Staphylococci and Streptococcus pyogenes were sensitive to the oil and Enterococcus faecalis, Streptococcus agalactiae were resistant. Among Gram –ve bacteria tested, only Pseudomonas aeruginosa was sensitive to oil and rest (Acinetobacter baumannii, Citrobacter freundii, Klebsiella pneumoniae, Proteus mirabilis, P. vulgaris and Vibrio cholerae) were insensitive. Out of 144 strains tested, most of which were resistant to a number of antibiotics, 97 were inhibited by the oil of black cumin. To the best of our knowledge, the activity of essential oil against coagulase negative Staphylococci (except S. epidermidis) and Streptococcus pyogenes is being reported for the first time.

Multi-drug resistance bacterial are a major public health concern in this post antibiotic era. The rate at which these bacteria are evolving is not synonymous with the rate at which new antibiotics are produce. In recent years, researches are being directed towards the use of herbal products in the treatment of various infections. One of such is the use of the Black seed cumin (Nigella sativa) in the treatment of ailments. In this study, different concentrations of Nigella sativa oil were tested for their antibacterial activity against different strains of Gram positive and Gram negative multi-drug resistant bacteria (MRSA, Acinetobacter baumannii, Escherichia coli, and Pseudomonas aeruginosa) by using well diffusion method. For all the different strains of Acinetobacter baumannii and E. coli that were tested against 100% of N. sativa oil, there was no recorded zone of inhibition. However, for the different strains of MRSA and Pseudomonas aeruginosa, different zones of inhibition where obtained for all the different oil dilutions used. Bacterial growth was inhibited at 100%, 80%, 50%, 40%, 30% and 20% N. sativa oil dilutions.

Journal of medicinal plant research

Chloroform and ethanol extracts of black cumin seeds (Nigella sativa) were analyzed for antibacterial activity against five food and water borne pathogens, namely, Staphylococcus aureus, Bacillus cereus, Vibrio cholerae, Klebsiella pneumoniae, Escherichia coli, and one food spoilage bacteria Bacillus subtilis, all of which were previously found to be resistant to different antibiotics. The antibacterial activities of the extracts were determined by disc diffusion and tube dilution methods. All the bacterial strains except E. coli and K. pneumoniae, showed sensitivity to the chloroform extract as well as the bacterial strains except the K. pneumoniae showed sensitivities to ethanol extract. The minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) of both extracts were also evaluated. Ethanol extract of black cumin was as found to be highly effective for B. subtilis (MIC value 375 μg/ml), followed by S. aureus (MIC value 1125 μg/ml). The highest concentration ...

2021

In Siddha medicine system has been classified into Aga marunthu (Internal) and Pura marunthu (External) having 32 forms of medicines in each. The external remedies are very useful for topical ulcers, skin diseases like eczema, psoriasis bacterial and fungal infections. The purpose this study was Evaluate the efficacy of Antibacterial activity of aqueous extract of Nigella sativa seeds powder against Staphylococcus aureus by used disk diffusion method. The result has been evaluated as the diameter of the zone of inhibition of microbial growth showed that the different concentration (100%, 50%, 25%) of Nigella sativa seed powder’s aqueous extract more effective against Staphylococcus aureus. Mean value of the hot extract 100%, 50%, 25% respectively 12.19±1.91mm, 7.63±2.32 mm and 3.78±1.73mm and the mean value of Cold extract were 9.45±1.32mm, 4.21±0.92mm and 2.71±.64mm. Standard maintained 18mm of zone of inhibition .Among the hot and cold extract, 100% of hot extract was showed more ...

Kurdistan Journal of Applied Research, 2022

Antimicrobial resistance is one of the most challenges ‎ that face the world today, and it is heading toward the post-antibiotic era. Traditionally, herbal extracts, medicinal plant oil extracts, and probiotics have been used as an alternative to antibiotics due to bacterial resistance and drug side effects. This ‎work is carried out to evaluate the antimicrobial efficacy of Nigella sativa oil extracts ‎against common resistant bacteria, including Methicillin-resistant staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. The black seed was purchased from the traditional herbal medicine market, Hot extraction of the oil was performed. The bacteria were obtained from a microbiology company and from clinical samples at the Shar hospital in Sulaimani Provence. The bacteria were gram-negative (Salmonella enterica, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive (methicillin-resistant Staphylococcus aureus (MRSA), Bacillus subtilis, and Bacillus cereus). The agar ‎dif...

2019

This study investigates the antimicrobial activity of essential oil obtained from Nigella sativa seeds (Black seed oil). The objectives are to determine the antimicrobial efficacy of black seed oil against selected pathogens such as Pseudomonas aeruginosa (ATCC 27853), Methicillin Resistant Staphylococcus aureus (MRSA), Methicillin sensitive Staphylococcus aureus (MSSA), Staphylococcus pyogenes (ATCC 19615), Escherichia coli (ATCC 25922 and ATCC 35218) and clinically isolated Candida albicans and to compare the ability of microbial growth inhibition with conventional antibiotics that are used for the test organisms. Bacteria were inoculated on Mueller Hinton Agar and Sabouraud Dextrose Agar was used to inoculate C. albicans. Agar well diffusion technique was used to evaluate antimicrobial activity of the test organisms. As Dimethyl sulfoxide (DMSO) was served as the solvent for black seed oil, it was used as the negative control. Among the test organisms, staphylococci; MRSA, MSSA, S. pyogenes and C. albicans were sensitive to the oil. Both two strains of E.coli, P. aeruginosa were resistant to the oil while all test organisms were susceptible to the antibiotics used. The comparative study revealed that all three staphylococci are more susceptible to black seed oil than amoxicillin in tested concentration.

Crude extracts of Nigella sativa L. (black cumin) seeds were tested in varying dilutions against strains of Bacillus cereus 2156, B. subtilis 2920, Escherichia coli 2065, Staphylococcus epidermidis 2493, Klebsiella pneumoniae 2957, Pseudomonas aeruginosa 5029, Salmonella typhmurium 2501, Enterobacter aerogens 5139 using agar well diffusion technique in swabbed Muellar Hinton agar plates under standard laboratory conditions. Extract in ethanol and n-hexane showed remarkable dose dependant antibacterial activity against the tested strains as evident from the zones of inhibition. No activity of the extract was observed against Pseudomonas aeruginosa 5029 and Enterobacter aerogens 5139. The most sensitive strain was S. epidermidis. No cross resistance was noticed with any of the tested antibiotics.