Mycobacterium tuberculosis

SeekTB- A two stage multiplex real-time PCR based method for the differentiation of the Mycobacterium tuberculosis complex

by Justin O'Grady

BioHealthBase: informatics support in the elucidation of influenza virus host pathogen interactions and virulence.

by R. Burke Squires

Arabinogalactan and lipoarabinomannan biosynthesis: structure, biogenesis and their potential as drug targets

by shipra grover

The ftsZ Gene of Mycobacterium smegmatis is expressed Through Multiple Transcripts

by Deepak Anand

Defining putative T cell epitopes from PE and PPE families of proteins of Mycobacterium tuberculosis with vaccine potential.

by Sridhar Hariharaputran

The identification of T cell epitopes from immune relevant antigens of Mycobacterium tuberculosis is a critical step... more The identification of T cell epitopes from immune relevant antigens of Mycobacterium tuberculosis is a critical step in the development of a vaccine covering diverse populations. Two multigene families, PE-PGRS and PPE make up about 10% of the M. tuberculosis genome. However, the functions of the proteins coded by these large numbers of genes are unknown. All possible nonameric peptide sequences from PE and PPE proteins were analysed in silico for their ability to bind to 33 alleles of class I HLA. These results reveal that of all PE and PPE proteins, a significant number of these peptides are predicted to be high-affinity HLA binders, irrespective of the length of the protein. The pathogen peptides that could behave as self or partially self-peptides in the host were eliminated using a comparative study with human proteome, thus reducing the number of peptides for analysis. The structural basis for recognition of the nonamers by the respective HLA molecules thus predicted was analyzed by molecular modeling. The structural analysis showed good correlation with the binding prediction. The analysis also led to an understanding of the binding profile of the peptides with respect to different alleles of class I HLA. The predicted epitopes can be tested experimentally for their inclusion in a potential vaccine against tuberculosis that is HLA haplotype-specific.

Construcción de un Laboratorio y un Animalario para trabajar con Mycobacterium tuberculosis

by Mayra Motta de Hodgson

Co-Autora con Carmen Lafoz Pueyo y María Peña Penabad

La tuberculosis (causada por M. tuberculosis) sigue siendo en la actualidad la enfermedad infecciosa más importante... more La tuberculosis (causada por M. tuberculosis) sigue siendo en la actualidad la enfermedad infecciosa más importante del mundo.
La transmisión de este agente se produce sobre todo por vía aérea. Por ello, es importante el establecimiento de normas estrictas a la hora de manejar este agente y establecer en las instalaciones el necesario nivel de bioseguridad.
En general se recomienda la utilización de instalaciones de nivel de bioseguridad 3 cuando se maneja este agente patógeno. Es por ello que nuestro proyecto ha tenido el objetivo de hacer una previsión del diseño, construcción y necesidades especiales de un laboratorio (BSL3) y un animalario (ABSL3) para el trabajo con este germen.
Para ello, en cada caso, se han descrito las necesidades estructurales, de materiales, de equipamiento y de mantenimiento, atendiendo a las características distintivas de cada uno de los dos locales y a sus posibilidades de integración y haciendo también mención a la validación de las instalaciones a fin de obra.
En fin, un proyecto ficticio, encaminado a reflejar de modo resumido los requerimientos previstos para este tipo de centros.

Improvement of the expression and purification of Mycobacterium tuberculosis arylamine N-acetyltransferase (TBNAT) a potential target for novel anti-tubercular …

by Areej Abuhammad

Analysis of [beta]-amino alcohols as inhibitors of the potential anti-tubercular target N-acetyltransferase

by Areej Abuhammad

Comparison of the Arylamine N-Acetyltransferase from Mycobacterium marinum and Mycobacterium tuberculosis

by Areej Abuhammad

Probing the architecture of the Mycobacterium marinum arylamine N-acetyltransferase active site

by Areej Abuhammad

BASTIDA,R., J.LOUREIRO, V.QUSE, A.BERNARDELLI, D.RODRIGUEZ y E.COSTA. (1999). Tuberculosis in a wild Subantarctic fur seal from Argentina. Journal of Wildlife Diseases, 35 (4): 796-798.

by Diego Rodriguez

A note reporting a tuberculosis case in Subantarctic fur seal (Arctocephalus tropicalis) vagrant off Argentina. A note reporting a tuberculosis case in Subantarctic fur seal (Arctocephalus tropicalis) vagrant off Argentina.

COUSINS,D.V, R.BASTIDA, A.CATALDI, V.QUSE, S.REDROBE, S.POW, P.DUIGNAN, A.MURRAY, N.AHMED, D.M.COLLINS, W.R.BUTLER, D.DAWSON, D.RODRIGUEZ, .LOUREIRO,M.I.ROMANO, A.ALITO, M.ZUMARRAGA and A.BERNARDELLI. 2003. Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp.nov. International Journal of Systematic and Evolutionary Microbiology, 53: 1305-1314.

by Diego Rodriguez

A comparison of Mycobacterium tuberculosis complex isolates from seals (pinnipeds) in Australia, Argentina, Uruguay,... more A comparison of Mycobacterium tuberculosis complex isolates from seals (pinnipeds) in Australia, Argentina, Uruguay, Great Britain and New Zealand was undertaken to determine their relationships to each other and their taxonomic position within the complex. Isolates from 30 cases of tuberculosis in six species of pinniped and seven related isolates were compared to representative and standard strains of the M. tuberculosis complex. The seal isolates could be distinguished from other members of the M. tuberculosis complex, including the recently defined ‘Mycobacterium canettii ’ and ‘Mycobacterium caprae’, on the basis of host preference and phenotypic and genetic tests. Pinnipeds appear to be the natural host for this ‘seal bacillus’, although the organism is also pathogenic in guinea pigs, rabbits, humans, Brazilian tapir (Tapirus terrestris) and, possibly, cattle. Infection caused by the seal bacillus is predominantly associated with granulomatous lesions in the peripheral lymph nodes, lungs, pleura, spleen and peritoneum. Cases of disseminated disease have been found. As with other members of the M. tuberculosis complex, aerosols are the most likely route of transmission. The name Mycobacterium pinnipedii sp. nov. is proposed for this novel member of the M. tuberculosis complex (the type strain is 6482T=ATCC BAA-688T=NCTC 13288T).

Tuberculosis at Spitalfields, London: an initial insight into medieval urban living

by Don Walker

Gray Jones, A. & Walker, D. Proceedings of the Sixth Annual Conference of the British Association for Biological Anthropology and Osteology. edited by Kate A. Robson Brown and Alice M. Roberts

A group of 10,500 skeletons were archaeologically excavated from the cemetery of the medieval hospital and priory of... more A group of 10,500 skeletons were archaeologically excavated from the cemetery of the medieval hospital and priory of St Mary-without-Bishopsgate (Spitalfields), London, England. The osteological analysis of the sample is ongoing until 2006 and this paper aims to report some of our initial findings, with particular reference to the prevalence of tuberculosis in the medieval population. In the sample of 1,654 skeletons studied so far, twenty-eight individuals were found to have the skeletal signs of tuberculosis (a crude prevalence rate of 1.7%). Initial trends suggest that the greatest prevalence was found in teenage and young adult individuals (12-25 years of age) and that more males were affected than females. The study of this un-paralleled sample from an urban medieval population has the potential to allow for a better understanding of tuberculosis throughout the entire life-course. In the future this bioarchaeological data, considered in combination with an un-rivalled understanding of the living conditions in medieval London, as derived from a wealth of literary and archaeological sources, will provide a unique opportunity to examine the prevalence and effects of this infectious disease within the urban environment.

Tuberculosis at Spitalfields, London: an initial insight into medieval urban living

by Don Walker

Gray Jones, A. & Walker, D. Proceedings of the Sixth Annual Conference of the British Association for Biological Anthropology and Osteology. edited by Kate A. Robson Brown and Alice M. Roberts

A group of 10,500 skeletons were archaeologically excavated from the cemetery of the medieval hospital and priory of... more A group of 10,500 skeletons were archaeologically excavated from the cemetery of the medieval hospital and priory of St Mary-without-Bishopsgate (Spitalfields), London, England. The osteological analysis of the sample is ongoing until 2006 and this paper aims to report some of our initial findings, with particular reference to the prevalence of tuberculosis in the medieval population. In the sample of 1,654 skeletons studied so far, twenty-eight individuals were found to have the skeletal signs of tuberculosis (a crude prevalence rate of 1.7%). Initial trends suggest that the greatest prevalence was found in teenage and young adult individuals (12-25 years of age) and that more males were affected than females. The study of this un-paralleled sample from an urban medieval population has the potential to allow for a better understanding of tuberculosis throughout the entire life-course. In the future this bioarchaeological data, considered in combination with an un-rivalled understanding of the living conditions in medieval London, as derived from a wealth of literary and archaeological sources, will provide a unique opportunity to examine the prevalence and effects of this infectious disease within the urban environment.

Construction of a severely attenuated mutant of Mycobacterium tuberculosis for reducing risk to laboratory workers

by Annemieke ten Bokum

Cholesterol utilization in mycobacteria is controlled by two TetR-type transcriptional regulators: kstR and kstR2

by Annemieke ten Bokum

Bystander macrophage apoptosis after Mycobacterium tuberculosis H37Ra infection

by Annemieke ten Bokum

Cholesteroid nature of free mycolic acids from M. tuberculosis

by Annemieke ten Bokum

The case for hypervirulence through gene deletion in Mycobacterium tuberculosis

by Annemieke ten Bokum

A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis

by Annemieke ten Bokum